Biochemistry

Enzymes: classification

"Over The HILL":

  • Oxidoreductases
  • Transferases
  • Hydrolases
  • Isomerases
  • Ligases
  • Lyases
  • Enzymes take  the reaction over the hill.

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Functions of Glutathione

 "T. M.E.D.I.C.S"

  • Transport of amino acids
  • Methemoglobin reduction
  • Erythrocyte homeostasis (inactivation of free radicals inside RBC)
  • Detoxification of organophosphorus, halogenated or nitrogenous compounds
  • Insulin Inactivation
  • Coenzyme in Reduction reactions
  • Scavanger of toxic substances
Glutathione was isolated in 1921 by Sir Frederick Hopkins and he won the Nobel prize in 1929.
As part of Gamma Glutamyl cycle (Miester cycle), glutathione plays a major role in transfer of neutral amino acids in intestines, renal tubules and brain.
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Purines & Pyrimidines

Purine = Pyrimidine + Imidazole ring

Purines are double ring structures and has pyrimidine ring in it fused to an imidazole ring.

Think: "Pyrimidines can be CUT out from purines!"

 Thus, Pyrimidines are

  • Cytosine
  • Uracil
  • Thiamine

 

Purines include Adenine, Guanine, Xanthine & Hypoxanthine.

 

 

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Isoenzymes

 

A. Isoenzymes of Creatinine Kinase (CK), 3 in number

  • CK1---BB--Brain
  • CK2---MB--Heart
  • CK3---MM--Sketal Muscles

 

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